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be derived from replicate arrays Most microarray experiments lack replicates due to the experimental costs When there are no replicates available, a better choice for imputation is to replace all of the missing data in an array with the average of valid expression values within the array The k-nearest-neighbor based method (KNN) does not demand experimental replicates Given a gene with missing gene expression readings, k genes with the most similar expression patterns (ie, its k neighbors) are found The given gene s missing values are imputed as the average expression values of its k neighbors [10], or predicted with the local least squares (LLS) method [11] Recent research has demonstrated that the weighted nearest-neighbors imputation method (WeNNI), in which both spot quality and correlations between genes were used in the imputation, is more effective than the traditional KNN method [12].

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They had talked about continuing their business interests by getting into the area of programming microprocessor computer systems, something they were well prepared for based on their Traf-O-Data experience They were excited about the new advances in microprocessor technology Surely, as these new gadgets became popular, there would be the chance to create a business opportunity Perhaps they could write a Basic interpreter for one of the new systems that must be about ready to be released Meanwhile, Gates had begun thinking about dropping out of Harvard In the summer of 1974, he interviewed for summer jobs at various places in the Boston area He and Allen wound up working together that summer, having taken jobs at Honeywell Corporation In December 1974, Allen came across the January 1975 issue of Popular Electronics.

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perhaps customised, is mailed out in summary, with the invitation to visit the Web site for more information (Figure 3.10). As can be seen, the opportunities for seductive graphics are limited, but this should not be a problem, as the intention is to get a short, snappy teaser onto the customer s PC. Brevity is important, as long messages are often not read and are felt to be an intrusion and consumption of eMail resources. The idea is to make it as easy as possible for the customer to then get to areas of speci c, in-depth interest. Note that Figure 3.10 shows two ways to do this. In the headline (the royal visit) the URL of the main site is simply given in at text. In the CORBA item, the Web address is given as a hot link, which can be clicked to give immediate access to the browser. This is only possible on some eMail systems. It can also be messy to use the full address of the page and it may be better to link to an intermediate contents page on the server.

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Domain knowledge can help estimate missing values based on the assumption that genes with similar biological functions have similar expression patterns Therefore, a missing value for a given gene can be estimated by evaluating the expression values of all genes that have the same or similar functions [13] Although such an approach is reasonable in terms of biology, its applicability is limited when the function is unknown for a large number of the genes In addition to the problems that are related to poor sample preparation, such as chip scratching or poor washing, a major source of problematic gene spots is relatively low signal intensity compared to background noise It is important to check the reasons for low signal intensity Gene expression might be very low, for instance, if the biological condition successfully blocks the gene expression.

12 On the cover of the magazine was a picture of the MITS Altair 8080, a rectangular metal machine with toggle switches and lights on the front..

that a breadth- rst search using parallel rule- ring will be appropriate Sometimes a depth- rst search is easier to visualize, as in MCFPS In general, since parallel rule- ring involves less systems overhead than sequential, a breadth- rst search is preferred if there is a choice 131 132 Defuzzi cation In both cases Fuzzi cation converts an input number into its word equivalents, and permits using words in the antecedent of rules red later on This is very useful for both numeric or word answers A discrete fuzzy set is a linguistic variable if its member words describe a number, such as fSmall, Medium, Largeg, and if membership functions de ned on the real line are used to map these words onto the real line It is not a linguistic variable if the words do not describe a number, such as fFord, Chevrolet, Chryslerg.

In this case, the low gene expression signal intensity is correct and the imputation of values estimated by the above-mentioned methods would probably produce a value that is too high An alternative is to replace such missing data with the lowest obtained intensity value within the same chip or with an arbitrary small number 423 Normalization.